P-234: Expression of Human Chorionic Gonadotropin (hCG) Hormone Using Chinese Hamster Ovary Cells

Background: Human chorionic gonadotropin (hCG) is a member of glycoprotein hormones family consist of two different non-covalently heterodimeric chains: alpha and beta subunits with 92 and 145 amino acids respectively. This hormone plays an important role in human reproduction and physiology especially for maintenance of the corpus luteum during the first months of pregnancy Materials and Methods: The aim of our study was to production of recombinant hCG in Chinese hamster ovary (CHO) cells. In this regard, cDNA encoding alpha and beta subunits were amplified by PCR and after digestion by XhoI/SalI cloned into the pcDNA3.1 (+) expression vector. The recombined plasmids were transformed into the TOP10F/ strains of E.coli. After that, cloning was confirmed by PCR and sequencing methods. Then two gene constructs were linearized by PvuI and subsequently co-transfected into the CHO cells using the electroporation Technique. The transfected cells were examined by PCR Results: Positive cell lines were subjected for expression analysis of recombinant hCG by SDS-PAGE and Western blotting methods Conclusion: Many researches confirm that production of recombinant protein in eukaryotic expression systems such as CHO cells is a reliable method for producing of therapeutic human chorionic gonadotropin